Reporter

Part:BBa_K4205001

Designed by: Xinyuan Wang   Group: iGEM22_NJMU-China   (2022-09-04)


PpbrA-PmerR+Lpp-OmpA-MT1A

PpbrA is a lead-specific promoter and PmerR is a mercury-specific promoter, and we constructed a OR gate by concatenating these two promoters. Either lead or mercury can turn on the expression of downstream genes.When expression of the recombinant protein is switched on, it is anchored to the outer surface of E. coli. At this time, MT1A will play the role of adsorption of heavy metals in the environment.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 58
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 58
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 58
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 584


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